Giants in sensing (accordingly to ACS Sensors journal)

Our recent collaborative work on pH-sensing DAOTA dyes (published in Analytical Chemistry in 2019) has been included into the virtual issue of ACS Sensors journal, celebrating 5 years and entitled 'Giants in sensing'.

To reflect back to what we have published over the period of 2015-2020 with ACS journals, see the list below:


Updates during COVID-19 pandemic

We are still alive and continue to work!

Over the past month of nation-wide work restrictions and subsequent lockdown our team did not stay idle and we participated in a number of research, collaborative and teaching activities.

- Shortly before the lockdown we finalised our new manuscript reporting new biosensor scaffold materials reporting extracellular calcium dynamics in 3D organoid model. The preprint version is available on BioRxiv.

- Together with Dr. M. Monaghan group (Trinity College Dublin) Dr. Dmitriev co-authored a review book chapter on optical imaging approaches employed in tissue engineering. This book chapter has been published as part of Springer Reference Series in Biomedical Engineering. The text (free to the members of TERMIS) is available here. Twitter link

- Members of the team in collaboration with Hubrecht Institute has prepared a book chapter for a book 'Intestinal Stem Cells', which is edited by Paloma Ordonez-Moran and will be published by Springer in July 2020. More information on this project can be found here.

- A number of new grant applications are in progress including recently submitted SFI/ EI/ IDA COVID-19 funding (decision pending). More details will be provided soon, if we get funded! 


New publication from the group: imaging of O2 nanosensors in implanted scaffold materials

Dr. R. Dmitriev performed and co-authored a collaborative study together with groups from Russia (Sechenov Institute for Regenerative Medicine and Privolzhsky Research Medical University) and Austria (TU Graz), which describes multi-parameter imaging of decellularized pericardial biomeshes in an implanted mouse model. One of highlights of the study is the finding that conjugated polymer-based O2 nanosensors can bind and remain bound to the biomeshes in live animal for extended period of time and thus used for subsequent O2 imaging in live animal and ex vivo. This confirms high usability of conjugated nanoparticle sensors previously developed by our team for such emerging applications as monitoring of tissue regeneration, tumour imaging and others.
The work has been published in Frontiers in Bioengineering and Biotechnology journal. Full text can be assessed here. Twitter link.

Biophysical Society 2020, San Diego

Dr. Ruslan Dmitriev recently presented a talk at the Biophysical Society 2020 Annual Meeting, in San Diego Convention Center, California, USA. In his talk, Ruslan summarised recent group developments in a multiparameter imaging of stem cell metabolism in the small intestinal organoid model. He focused on measuring oxygenation of live intestinal organoids, assessing mitochondrial polarisation and combining O2 with NAD(P)H redox microscopy analyses.
He also received Travel Award from the Biophysical Society to attend this meeting.
More information on the meeting can be found on Twitter
The abstract of presentation published in Biophysical Journal supplement can be viewed here.


Interview to Biotechniques journal

During the ASCB-EMBO 2019 conference in Washington DC (December 2019) Dr. Ruslan Dmitriev was interviewed by Biotechniques journal about FLIM, organoids, challenges and career inspirations. Here's an interview:


First paper from 2020!

Our manuscript on analysis of Lgr5-GFP intestinal organoid metabolism by different methods (O2-PLIM, NAD(P)H-FLIM and XF assay) has been accepted for publication by Redox Biology journal. This is an excellent result of collaboration with Prof. Michael Monaghan group at Trinity College Dublin (NAD(P)H-FLIM), which enabled us to compare and efficiently use 2 different FLIM-PLIM imaging platforms available in Ireland. Combining these innovative experimental approaches enabled us to discover highly 'flexible' and dynamic ability of stem cells to respond to the nutrient availability in the growth media. The next stages of our research on stem cell niche metabolism promise to be even more exciting!

More on this story:

The story about our FLIM method was highlighted in Russian University media

The webpage of Sechenov First Medical University (Moscow, Russia) has highlighted our recent article on analysis of the mitochondrial membrane potential by FLIM method (published in autumn 2019).

The media article (in Russian) can be found here.